2F35
Crystal Structure of the GluR5 Ligand Binding Core with UBP302 At 1.87 Angstroms Resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-002 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2005-08-28 |
| Detector | MARRESEARCH |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 97.876, 97.907, 128.347 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 38.920 - 1.870 |
| R-factor | 0.19273 |
| Rwork | 0.191 |
| R-free | 0.22036 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | in process UBP310 |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.457 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 1.930 |
| High resolution limit [Å] | 1.860 | 1.860 |
| Rmerge | 0.057 | 0.430 |
| Number of reflections | 52211 | |
| <I/σ(I)> | 16.3 | |
| Completeness [%] | 98.6 | 85.7 |
| Redundancy | 7.1 | 6.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.9 | 293 | 20% PEG 1K 100 mM Tris 5 mM UBP302, pH 8.9, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
