2F31
Crystal structure of the autoinhibitory switch in Formin mDia1; the DID/DAD complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RU300 |
Temperature [K] | 200 |
Detector technology | AREA DETECTOR |
Collection date | 2005-05-03 |
Detector | MARRESEARCH |
Wavelength(s) | 1.54180 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 102.160, 65.790, 46.860 |
Unit cell angles | 90.00, 104.08, 90.00 |
Refinement procedure
Resolution | 7.970 - 2.100 |
R-factor | 0.2328 |
Rwork | 0.229 |
R-free | 0.31170 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.006 |
RMSD bond angle | 1.556 |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | REFMAC |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 8.000 | 2.200 |
High resolution limit [Å] | 2.050 | 2.050 |
Rmerge | 0.058 | 0.347 |
Number of reflections | 16811 | |
<I/σ(I)> | 11.54 | 2.93 |
Completeness [%] | 84.8 | 83.6 |
Redundancy | 2.14 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6 | 293 | 0.1 M sodium citrate, 200 mM ammonium sulfate, 25% PEG 4000, pH 6.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K |