2F2W
alpha-spectrin SH3 domain R21A mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE BW7B |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | BW7B |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2004-05-30 |
| Detector | MACSCIENCE |
| Wavelength(s) | 0.8414 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 32.906, 42.111, 47.937 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.370 - 1.700 |
| R-factor | 0.203 |
| Rwork | 0.203 |
| R-free | 0.24600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1shg |
| RMSD bond length | 0.004 |
| RMSD bond angle | 1.100 |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((SCALA)) |
| Phasing software | CNS |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.790 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.096 | |
| Number of reflections | 6723 | |
| <I/σ(I)> | 5.5 | |
| Completeness [%] | 89.2 | 89.2 |
| Redundancy | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 3 | 298 | Ammonium sulphate 2M, pH 3, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
