2F2V
alpha-spectrin SH3 domain A56G mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | BRUKER AXS MICROSTAR |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-10-05 |
| Detector | BRUKER PROTEUM X8 |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 23.932, 41.617, 45.981 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 1.850 |
| R-factor | 0.17526 |
| Rwork | 0.173 |
| R-free | 0.22324 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1shg |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.248 |
| Data reduction software | SAINT |
| Data scaling software | SADABS |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 1.950 |
| High resolution limit [Å] | 1.850 | 1.850 |
| Rmerge | 0.027 | 0.079 |
| Number of reflections | 3983 | |
| <I/σ(I)> | 31.37 | 11.5 |
| Completeness [%] | 93.7 | 70.4 |
| Redundancy | 3.32 | 1.17 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 288 | 2M Sodium Formiate, 0.1 M MES, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 288K |
