2F2P

Structure of calmodulin bound to a calcineurin peptide: a new way of making an old binding mode

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X8C
Synchrotron site	NSLS
Beamline	X8C
Temperature [K]	100
Spacegroup name	P 3 2 1
Unit cell lengths	139.549, 139.549, 45.592
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	50.000 - 2.600
R-factor	0.24
Rwork	0.234
R-free	0.29700
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1cdm
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	CNS (1.1)

Data quality characteristics

	Overall
Low resolution limit [Å]	119.523
High resolution limit [Å]	2.600
Number of reflections	15901
Completeness [%]	99.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	VAPOR DIFFUSION, HANGING DROP, temperature 298K