2F2N

Triclinic hen egg lysozyme cross-linked by glutaraldehyde

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	LURE BEAMLINE DW32
Synchrotron site	LURE
Beamline	DW32
Temperature [K]	277
Detector technology	IMAGE PLATE
Collection date	2000-01-01
Detector	MARRESEARCH
Wavelength(s)	0.974
Spacegroup name	P 1
Unit cell lengths	27.170, 31.840, 34.220
Unit cell angles	88.67, 108.32, 111.87

Refinement procedure

Resolution	8.000 - 1.603
R-factor	0.1375
Rwork	0.137
R-free	0.16910
Structure solution method	FOURIER SYNTHESIS
Starting model (for MR)	3lzt 1lks
RMSD bond length	0.010
RMSD bond angle	0.026
Data reduction software	MOSFLM
Data scaling software	SCALA
Refinement software	SHELXL-97

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	8.700	1.700
High resolution limit [Å]	1.603	1.603
Rmerge	0.045	0.174
Number of reflections	12315
<I/σ(I)>	32.01	17.2
Completeness [%]	93.5	87.84
Redundancy	4.3	3.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	300	sodium nitrate, pH 6, VAPOR DIFFUSION, HANGING DROP, temperature 300K