2F23

Crystal structure of GreA factor homolog 1 (Gfh1) protein of Thermus thermophilus

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X4A
Synchrotron site	NSLS
Beamline	X4A
Temperature [K]	100
Detector technology	CCD
Collection date	2003-08-26
Detector	ADSC QUANTUM 4r
Wavelength(s)	0.97917, 0.97939, 0.97853
Spacegroup name	P 1 21 1
Unit cell lengths	28.412, 152.662, 32.375
Unit cell angles	90.00, 102.65, 90.00

Refinement procedure

Resolution	27.720 - 1.600
Rwork	0.206
R-free	0.25400
Structure solution method	MAD
RMSD bond length	0.005
RMSD bond angle	1.200
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHASES
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	38.170	1.570
High resolution limit [Å]	1.520	1.520
Rmerge	0.098	0.312
Number of reflections	39911
Completeness [%]	96.7	87.5
Redundancy	7.1	5.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	296	29% PEG 3350, 0.1M MES, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 296K
1	VAPOR DIFFUSION, HANGING DROP	6	296	29% PEG 3350, 0.1M MES, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 296K
1	VAPOR DIFFUSION, HANGING DROP	6	296	29% PEG 3350, 0.1M MES, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 296K