2F1W

Crystal structure of the TRAF-like domain of HAUSP/USP7

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X25
Synchrotron site	NSLS
Beamline	X25
Temperature [K]	100
Detector technology	CCD
Collection date	2004-07-01
Detector	ADSC QUANTUM 4
Wavelength(s)	1.1, 0.9794, 0.9797, 0.9500
Spacegroup name	P 21 21 21
Unit cell lengths	45.172, 52.242, 63.651
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 1.650
R-factor	0.195
Rwork	0.194
R-free	0.21200
Structure solution method	MAD
RMSD bond length	0.009
RMSD bond angle	1.560
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SOLVE
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	99.000	1.680
High resolution limit [Å]	1.600	1.600
Number of reflections	20180
Completeness [%]	1.0	60

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	285	240 mM CaCl2, 6.5% PEG4000, 20 mM ammonium sulfate, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 285K