2F1V
Outer membrane protein OmpW
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X6A |
| Synchrotron site | NSLS |
| Beamline | X6A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-06-30 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 1.10 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 118.666, 118.776, 118.519 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 8.000 - 2.700 |
| R-factor | 0.293 |
| Rwork | 0.292 |
| R-free | 0.31400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2f1t MOL_A |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.600 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER ((CCP4)) |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 37.560 | 2.690 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.087 | 0.716 |
| Number of reflections | 52992 | |
| <I/σ(I)> | 25.7 | 2.25 |
| Completeness [%] | 100.0 | 82.1 |
| Redundancy | 7.3 | 6.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 295 | 27-33% MPD, 0.2 M ammonium acetate, 50 mM sodium citrate, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
