2F1T
Outer membrane protein OmpW
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X6A |
| Synchrotron site | NSLS |
| Beamline | X6A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-06-29 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 1.10 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 82.158, 82.158, 186.234 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 8.000 - 3.000 |
| R-factor | 0.276 |
| Rwork | 0.274 |
| R-free | 0.32900 |
| Structure solution method | SIRAS |
| Starting model (for MR) | none |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.600 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | SHARP |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.800 | 2.540 |
| High resolution limit [Å] | 2.450 | 2.450 |
| Rmerge | 0.067 | 0.774 |
| Number of reflections | 14157 | |
| <I/σ(I)> | 38.4 | 2.1 |
| Completeness [%] | 98.7 | 98 |
| Redundancy | 9 | 6.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 9 | 295 | 28-32% PEG400, 0.2 M CaCl2, 50 mM glycine, pH 9, VAPOR DIFFUSION, temperature 295K |
| 1 | VAPOR DIFFUSION | 9 | 295 | 28-32% PEG400, 0.2 M CaCl2, 50 mM glycine, pH 9, VAPOR DIFFUSION, temperature 295K |
