2F1S
Crystal Structure of a Viral FLIP MC159
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X25 |
| Synchrotron site | NSLS |
| Beamline | X25 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-02-01 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.979, 0.9794, 0.98, 0.9641 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 34.859, 63.271, 76.360 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 1.400 |
| R-factor | 0.206 |
| Rwork | 0.206 |
| R-free | 0.23600 |
| Structure solution method | MAD |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.620 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.450 |
| High resolution limit [Å] | 1.400 | 1.400 |
| Number of reflections | 33495 | |
| Completeness [%] | 98.0 | 93 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | PEG 2000, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
