2F1R
Crystal Structure of molybdopterin-guanine biosynthesis protein B (mobB)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X25 |
| Synchrotron site | NSLS |
| Beamline | X25 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-09-06 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 37.980, 63.930, 66.010 |
| Unit cell angles | 90.00, 95.30, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.100 |
| R-factor | 0.1912 |
| Rwork | 0.186 |
| R-free | 0.25340 |
| Structure solution method | SAD |
| RMSD bond length | 0.021 |
| RMSD bond angle | 1.854 |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.180 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.099 | 0.172 |
| Number of reflections | 17059 | |
| <I/σ(I)> | 14.4 | |
| Completeness [%] | 92.1 | 43.5 |
| Redundancy | 9.8 | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.51 | 298 | 20% Peg 3350, 0.1M Bis-Tris, additive 0.1M Praseodymium (III) acetate, pH 5.51, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
