2F1L
CRYSTAL STRUCTURE OF A PUTATIVE 16S RIBOSOMAL RNA PROCESSING PROTEIN RIMM (PA3744) FROM PSEUDOMONAS AERUGINOSA AT 2.46 A RESOLUTION
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL9-2 |
| Synchrotron site | SSRL |
| Beamline | BL9-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-07-04 |
| Detector | MARMOSAIC 325 mm CCD |
| Wavelength(s) | 0.97932, 0.91162, 0.97915 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 80.228, 80.228, 71.855 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 26.800 - 2.460 |
| R-factor | 0.17732 |
| Rwork | 0.175 |
| R-free | 0.23700 |
| Structure solution method | MAD |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.702 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 26.760 | 26.760 | 2.520 |
| High resolution limit [Å] | 2.400 | 11.000 | 2.460 |
| Rmerge | 0.079 | 0.061 | 0.752 |
| Number of reflections | 9930 | ||
| <I/σ(I)> | 6 | 8 | 1 |
| Completeness [%] | 98.9 | 94.3 | 88.4 |
| Redundancy | 5.4 | 4.1 | 4.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 4.6 | 293 | 30.0% Glycerol, 5.6% PEG-4000, 0.1M Acetate, pH 4.6, VAPOR DIFFUSION, SITTING DROP, NANODROP, temperature 293K |
