2F1I

Recombinase in Complex with AMP-PNP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	ENRAF-NONIUS
Temperature [K]	100
Detector technology	CCD
Collection date	2004-01-16
Detector	ENRAF-NONIUS
Wavelength(s)	1.5418
Spacegroup name	P 61
Unit cell lengths	83.458, 83.458, 106.787
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	20.000 - 2.900
R-factor	0.211
Rwork	0.209
R-free	0.25600
Structure solution method	FOURIER SYNTHESIS
Starting model (for MR)	1t4g
RMSD bond length	0.008
RMSD bond angle	1.270
Data reduction software	LSCALE
Data scaling software	LSCALE
Phasing software	AMoRE
Refinement software	CNS (1.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	3.000
High resolution limit [Å]	2.900	2.900
Number of reflections	9401
<I/σ(I)>	7.2	1.6
Completeness [%]		98.1
Redundancy	14.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	293	0.05M MAGNESIUM CHLORIDE, 0.5 M NaCl, 0.05M TRIS, 6% PEG 3350, pH 7.50, VAPOR DIFFUSION, HANGING DROP, temperature 293.0K