2F1H

RECOMBINASE IN COMPLEX WITH AMP-PNP and Potassium

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	ENRAF-NONIUS
Temperature [K]	100
Detector technology	CCD
Collection date	2004-11-20
Detector	ENRAF-NONIUS
Wavelength(s)	1.5418
Spacegroup name	P 61
Unit cell lengths	84.453, 84.453, 105.484
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	20.000 - 2.700
R-factor	0.227
Rwork	0.227
R-free	0.29700
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1pzn
RMSD bond length	0.008
RMSD bond angle	1.300
Data reduction software	LSCALE
Data scaling software	LSCALE
Phasing software	AMoRE
Refinement software	CNS (1.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.800
High resolution limit [Å]	2.700	2.700
Number of reflections	11119
<I/σ(I)>	7.6
Completeness [%]		94.2
Redundancy	4.5	4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	293	0.05M MAGNESIUM CHLORIDE, 0.5 M KCl, 0.05 M TRIS, 6% PEG 3350, pH 7.50, VAPOR DIFFUSION, HANGING DROP, temperature 293.0K