2F1F
Crystal structure of the regulatory subunit of acetohydroxyacid synthase isozyme III from E. coli
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID14-4 |
Synchrotron site | ESRF |
Beamline | ID14-4 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2004-11-30 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.97924, 0.97942, 0.93928 |
Spacegroup name | I 4 |
Unit cell lengths | 98.600, 98.600, 80.000 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 20.000 - 1.750 |
R-factor | 0.174 |
Rwork | 0.171 |
R-free | 0.22700 |
Structure solution method | MAD |
RMSD bond length | 0.025 |
RMSD bond angle | 2.118 |
Data scaling software | XSCALE |
Phasing software | SHARP |
Refinement software | REFMAC |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.000 | 1.800 |
High resolution limit [Å] | 1.750 | 1.750 |
Rmerge | 0.063 | 0.541 |
Number of reflections | 38313 | 2905 |
<I/σ(I)> | 15.1 | 3.3 |
Completeness [%] | 99.2 | 100 |
Redundancy | 6.15 | 6.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 8.5 | 293 | 10-25 mg/ml protein in 0.5 M MgCl2, 1:1 mixed with reservoir made up of 30-40% PEG400, 0.4-0.6 M MgCl2, 100 mM Tris-HCl pH 8.5, VAPOR DIFFUSION, temperature 293K |
1 | VAPOR DIFFUSION | 8.5 | 293 | 10-25 mg/ml protein in 0.5 M MgCl2, 1:1 mixed with reservoir made up of 30-40% PEG400, 0.4-0.6 M MgCl2, 100 mM Tris-HCl pH 8.5, VAPOR DIFFUSION, temperature 293K |
1 | VAPOR DIFFUSION | 8.5 | 293 | 10-25 mg/ml protein in 0.5 M MgCl2, 1:1 mixed with reservoir made up of 30-40% PEG400, 0.4-0.6 M MgCl2, 100 mM Tris-HCl pH 8.5, VAPOR DIFFUSION, temperature 293K |