2F0R
Crystallographic structure of human Tsg101 UEV domain
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | BRUKER AXS MICROSTAR |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2005-10-05 |
Detector | BRUKER PROTEUM X8 |
Wavelength(s) | 1.54 |
Spacegroup name | H 3 |
Unit cell lengths | 97.865, 97.865, 110.583 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 30.000 - 2.260 |
R-factor | 0.19059 |
Rwork | 0.188 |
R-free | 0.24271 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1s1q |
RMSD bond length | 0.020 |
RMSD bond angle | 1.785 |
Data reduction software | SAINT |
Data scaling software | CCP4 ((SCALA)) |
Phasing software | MOLREP |
Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 60.000 | 2.314 |
High resolution limit [Å] | 2.250 | 2.255 |
Rmerge | 0.030 | |
Number of reflections | 17160 | |
<I/σ(I)> | 26.3 | |
Completeness [%] | 92.4 | 91.73 |
Redundancy | 5.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8 | 288 | 20% PEG 4000, 0.2 M ammonium sulphate, 0.1 M Tris , pH 8.0, VAPOR DIFFUSION, SITTING DROP, temperature 288K |