2F0C
Structure of the Receptor Binding Protein (ORF49, bbp) from lactophage tp901-1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-3 |
| Synchrotron site | ESRF |
| Beamline | ID14-3 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-10-27 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.931 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 72.464, 78.716, 81.656 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 40.000 - 1.650 |
| R-factor | 0.15802 |
| Rwork | 0.156 |
| R-free | 0.18874 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2BSD residues 162-264 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.286 |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((SCALA)) |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 81.000 | 1.740 |
| High resolution limit [Å] | 1.650 | 1.650 |
| Rmerge | 0.074 | 0.282 |
| Number of reflections | 56893 | |
| <I/σ(I)> | 7.4 | 2.6 |
| Completeness [%] | 100.0 | 100 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 293 | 1 M ammonium sulfate, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
