2F0A

Crystal Structure of Monomeric Uncomplexed form of Xenopus dishevelled PDZ domain

Experimental procedure

実験手法	MAD
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.2
Synchrotron site	ALS
Beamline	5.0.2
Temperature [K]	100
Detector technology	CCD
Collection date	2002-08-01
Detector	ADSC QUANTUM 4
Wavelength(s)	0.9798, 0.9801, 0.9611
Spacegroup name	P 61
格子定数 [Å]	89.834, 89.834, 82.471
格子定数 [度]	90.00, 90.00, 120.00

精密化法

残基	19.450 - 1.800
R因子	0.21571
Rwork	0.214
R-free	0.25263
Structure solution method	MAD
結合長の平均二乗偏差(RMSD) [Å]	0.019
結合角の平均二乗偏差(RMSD) [度]	1.696
Data scaling software	HKL-2000
Phasing software	SOLVE
Refinement software	REFMAC (5.2.0005)

Quality characteristics

	Overall
分解能 [Å] (低)	19.450
分解能 [Å] (高)	1.800
独立反射数	34969
完全性 [%]	100.0

結晶化条件

結晶ID	方法	pH	温度	溶液条件
1	VAPOR DIFFUSION, HANGING DROP	6.7	297	1.4 M ammonium sulfate 0.1 M sodium cacodylate, pH 6.7, VAPOR DIFFUSION, HANGING DROP, temperature 297K