2F08
Crystal structure of a major house dust mite allergen, Derf 2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | MACSCIENCE |
| Temperature [K] | 298 |
| Detector technology | AREA DETECTOR |
| Collection date | 1999-12-21 |
| Detector | SIEMENS HI-STAR |
| Wavelength(s) | 1.54 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 106.124, 54.328, 102.354 |
| Unit cell angles | 90.00, 91.35, 90.00 |
Refinement procedure
| Resolution | 14.910 - 2.200 |
| R-factor | 0.206 |
| Rwork | 0.206 |
| R-free | 0.24500 |
| Structure solution method | MIR |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.300 |
| Data reduction software | SAINT |
| Data scaling software | SAINT |
| Phasing software | PHASES |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 28.300 | 2.175 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Number of reflections | 32658 | |
| <I/σ(I)> | 25.83 | 2.61 |
| Completeness [%] | 95.0 | 99.1 |
| Redundancy | 3.38 | 3.37 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 23% PEG 4000, 0.06M ammonium sulfate, 0.1M sodium acetate, 10mM HEPES , pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
