2F07

Crystal Structure of YvdT from Bacillus subtilis

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	BSRF BEAMLINE 3W1A
Synchrotron site	BSRF
Beamline	3W1A
Temperature [K]	100
Detector technology	CCD
Collection date	2004-01-01
Detector	MARRESEARCH
Wavelength(s)	0.98017
Spacegroup name	P 65
Unit cell lengths	115.608, 115.608, 97.032
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	20.000 - 2.300
R-factor	0.238
Rwork	0.234
R-free	0.25500
Structure solution method	SAD
RMSD bond length	0.008
RMSD bond angle	1.300
Data scaling software	SCALEPACK
Phasing software	SHELXS
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.380
High resolution limit [Å]	2.300	2.300
Number of reflections	31374
<I/σ(I)>	22.04	6.98
Completeness [%]	99.8	100
Redundancy	6.99

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.5	291	0.1M Bis-Tris pH5.5, 2.1M Ammonium Sulfate, VAPOR DIFFUSION, HANGING DROP, temperature 291K