2F06
Crystal structure of protein BT0572 from Bacteroides thetaiotaomicron
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-10-06 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97940 0.97954, 0.97172 |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 64.622, 64.622, 244.942 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 50.000 - 2.100 |
| R-factor | 0.20481 |
| Rwork | 0.203 |
| R-free | 0.24941 |
| Structure solution method | MAD |
| RMSD bond length | 0.026 |
| RMSD bond angle | 2.253 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | HKL-3000 |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.180 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.086 | 0.476 |
| Number of reflections | 18739 | |
| <I/σ(I)> | 60.1 | 4.3 |
| Completeness [%] | 99.6 | 98.7 |
| Redundancy | 21.3 | 15.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 291 | 8% PEG 6000, 0.1M Mes pH 6.5, 20% glycerol, VAPOR DIFFUSION, SITTING DROP, temperature 291K |
