2EZ2
Apo tyrosine phenol-lyase from Citrobacter freundii at pH 8.0
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE BM14 |
Synchrotron site | ESRF |
Beamline | BM14 |
Temperature [K] | 120 |
Detector technology | CCD |
Collection date | 2002-05-13 |
Detector | MARRESEARCH |
Wavelength(s) | 1.00 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 133.644, 143.735, 59.915 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 16.740 - 1.850 |
R-factor | 0.175 |
Rwork | 0.174 |
R-free | 0.20600 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.015 |
RMSD bond angle | 1.414 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | REFMAC (5.2) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 17.000 | 1.880 |
High resolution limit [Å] | 1.850 | 1.850 |
Rmerge | 0.055 | 0.381 |
Number of reflections | 95091 | |
Completeness [%] | 95.5 | 85.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 293 | 50 mM potassium phosphate, 2 mM DDT, 0.2 M KCl, 32.5% (w/v) monomethyl ether PEG 2000, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |