2EZ1
Holo tyrosine phenol-lyase from Citrobacter freundii at pH 8.0
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE BW7B |
| Synchrotron site | EMBL/DESY, Hamburg |
| Beamline | BW7B |
| Temperature [K] | 120 |
| Detector technology | IMAGE PLATE |
| Collection date | 1995-06-22 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.87 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 133.860, 143.850, 60.070 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.840 - 1.900 |
| R-factor | 0.153 |
| Rwork | 0.152 |
| R-free | 0.18600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.312 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.930 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.075 | 0.359 |
| Number of reflections | 85710 | |
| Completeness [%] | 92.0 | 90.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOUR DIFFUSION, HANGING DROP | 8 | 293 | 50 mM triethanolamine buffer, 0.5 mM PLP, 2 mM DDT, 0.4 M to 0.8 M KCl, 35 - 38% (w/v) monomethyl ether PEG 5000, pH 8.0, VAPOUR DIFFUSION, HANGING DROP, temperature 293K |
