2EZ0
Crystal structure of the S107A/E148Q/Y445A mutant of EcClC, in complex with a FaB fragment
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-02-14 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.91931 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 220.155, 124.032, 149.282 |
| Unit cell angles | 90.00, 127.97, 90.00 |
Refinement procedure
| Resolution | 19.920 - 3.540 |
| R-factor | 0.258 |
| Rwork | 0.258 |
| R-free | 0.29600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1otu |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.600 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.620 |
| High resolution limit [Å] | 3.500 | 3.500 |
| Number of reflections | 33492 | |
| Completeness [%] | 89.1 | 90.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 9.5 | 293 | 36% peg200/300 1:2, 100mM NaBr, 50 mM NaKTart, 50 mM Glycine, pH 9.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
