2EY4
Crystal Structure of a Cbf5-Nop10-Gar1 Complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 173 |
| Detector technology | CCD |
| Collection date | 2005-03-26 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 1 |
| Unit cell lengths | 36.087, 75.361, 103.240 |
| Unit cell angles | 95.90, 96.90, 94.99 |
Refinement procedure
| Resolution | 39.970 - 2.110 |
| R-factor | 0.211 |
| Rwork | 0.211 |
| R-free | 0.25500 |
| Structure solution method | MAD |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.300 |
| Data reduction software | bioteX |
| Phasing software | CNS |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | |
| High resolution limit [Å] | 2.100 | 2.100 |
| Number of reflections | 51837 | |
| Completeness [%] | 55.6 | 95 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 6.5 | 298 | In hanging drops, pH 6.5, VAPOR DIFFUSION, temperature 298.0K |
| 1 | VAPOR DIFFUSION | 6.5 | 298 | In hanging drops, pH 6.5, VAPOR DIFFUSION, temperature 298.0K |
| 1 | VAPOR DIFFUSION | 6.5 | 298 | In hanging drops, pH 6.5, VAPOR DIFFUSION, temperature 298.0K |
