2EXY
Crystal structure of the E148Q Mutant of EcClC, Fab complexed in absence of bound ions
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-04-23 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.919998 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 219.640, 119.788, 151.300 |
| Unit cell angles | 90.00, 128.09, 90.00 |
Refinement procedure
| Resolution | 19.970 - 3.100 |
| R-factor | 0.328 |
| Rwork | 0.328 |
| R-free | 0.35200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1otu |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.500 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.160 |
| High resolution limit [Å] | 3.100 | 3.050 |
| Number of reflections | 58313 | |
| Completeness [%] | 97.5 | 93.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 293 | peg 200/300 1:2, 150mM Tart, 50mM Tris, pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
