2EXX
Crystal structure of HSCARG from Homo sapiens in complex with NADP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-BM |
| Synchrotron site | APS |
| Beamline | 22-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-08-06 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.97928 |
| Spacegroup name | F 2 3 |
| Unit cell lengths | 223.300, 223.300, 223.300 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 - 2.400 |
| R-factor | 0.235 |
| Rwork | 0.233 |
| R-free | 0.26500 |
| Structure solution method | SAD |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SHARP |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.490 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Number of reflections | 34021 | |
| Completeness [%] | 95.3 | 100 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 277 | 1.2M sodium potassium phosphate, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
