2EXR

X-Ray Structure of Cytokinin Oxidase/Dehydrogenase (CKX) From Arabidopsis Thaliana AT5G21482

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2005-10-10
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	0.97911, 0.96403
Spacegroup name	C 2 2 21
Unit cell lengths	45.858, 114.502, 190.920
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	42.570 - 1.702
R-factor	0.17331
Rwork	0.171
R-free	0.21390
Structure solution method	MAD
RMSD bond length	0.014
RMSD bond angle	1.392
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SHARP
Refinement software	REFMAC (refmac_5.2.0005)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	42.570	1.760
High resolution limit [Å]	1.700	1.700
Rmerge	0.091	0.232
Number of reflections	52961
<I/σ(I)>	10.709	4.373
Completeness [%]	95.1	73.4
Redundancy	6.8	4.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	277	Protein solution (10 mg/ml protein, 0.10 M Bis Tris, 0.050 M sodium chloride, 0.0031 M sodium azide, 0.0003 M TCEP, pH 7.0) mixed in a 1:1 ratio with the well solution (24% MEPEG 5K, 0.05 M magnesium sulfate, 0.1 M HEPPS pH 8.5),temperature 277K, vapor diffusion, hanging drop
1	VAPOR DIFFUSION, HANGING DROP	8.5	277	Protein solution (10 mg/ml protein, 0.10 M Bis Tris, 0.050 M sodium chloride, 0.0031 M sodium azide, 0.0003 M TCEP, pH 7.0) mixed in a 1:1 ratio with the well solution (24% MEPEG 5K, 0.05 M magnesium sulfate, 0.1 M HEPPS pH 8.5),temperature 277K, vapor diffusion, hanging drop