2EXK

Structure of the family43 beta-Xylosidase E187G from geobacillus stearothermophilus in complex with xylobiose

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	EMBL/DESY, HAMBURG BEAMLINE X13
Synchrotron site	EMBL/DESY, HAMBURG
Beamline	X13
Temperature [K]	100
Detector technology	CCD
Collection date	2005-05-26
Detector	MARRESEARCH
Wavelength(s)	0.8048
Spacegroup name	P 43 21 2
Unit cell lengths	139.870, 139.870, 232.025
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	2.230 - 2.200
R-factor	0.188
Rwork	0.185
R-free	0.23800
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.010
RMSD bond angle	1.340
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	REFMAC

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.000	2.230
High resolution limit [Å]	2.200	2.200
Number of reflections	116620
Completeness [%]	99.5	97.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.4	285	17% PEG 6000, 0.1M MES, pH 5.4, VAPOR DIFFUSION, SITTING DROP, temperature 285K