2EXH

Structure of the family43 beta-Xylosidase from geobacillus stearothermophilus

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	EMBL/DESY, HAMBURG BEAMLINE BW7B
Synchrotron site	EMBL/DESY, HAMBURG
Beamline	BW7B
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2005-07-29
Detector	MARRESEARCH
Wavelength(s)	0.8426
Spacegroup name	P 43 21 2
Unit cell lengths	140.027, 140.027, 231.599
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	40.000 - 1.880
R-factor	0.235
Rwork	0.233
R-free	0.28600
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	1.299
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	REFMAC

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.000	1.910
High resolution limit [Å]	1.880	1.880
Number of reflections	187065
Completeness [%]	99.3	90.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.4	285	17% PEG 6000, 0.1M MES, pH 5.4, VAPOR DIFFUSION, SITTING DROP, temperature 285K