2EX0
Crystal structure of multifunctional sialyltransferase from Pasteurella Multocida
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL9-2 |
| Synchrotron site | SSRL |
| Beamline | BL9-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-06-03 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.9800 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 63.718, 61.281, 96.649 |
| Unit cell angles | 90.00, 101.56, 90.00 |
Refinement procedure
| Resolution | 43.730 - 1.650 |
| R-factor | 0.193 |
| Rwork | 0.193 |
| R-free | 0.21800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.200 |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((SCALA)) |
| Phasing software | EPMR |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 62.380 | 1.690 |
| High resolution limit [Å] | 1.650 | 1.650 |
| Rmerge | 0.052 | 0.423 |
| Number of reflections | 86251 | |
| <I/σ(I)> | 15.1 | 2.5 |
| Completeness [%] | 98.1 | 82.1 |
| Redundancy | 3.3 | 2.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 298 | 0.216 mM protein, 26% PEG3350, 0.1M NaCl, 0.4% Triton-X-100, 0.1M HEPES, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
