2EWW
Crystal Structure of the Pilus Retraction Motor PilT and Bound ATP
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 14-ID-B |
Synchrotron site | APS |
Beamline | 14-ID-B |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2003-11-01 |
Detector | MARRESEARCH |
Wavelength(s) | 0.9794 |
Spacegroup name | P 6 |
Unit cell lengths | 107.380, 107.380, 68.860 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 19.470 - 3.200 |
R-factor | 0.24236 |
Rwork | 0.239 |
R-free | 0.30981 |
Structure solution method | MAD |
RMSD bond length | 0.007 |
RMSD bond angle | 1.149 |
Data reduction software | MOSFLM |
Data scaling software | CCP4 ((SCALA)) |
Phasing software | SHARP |
Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 3.370 |
High resolution limit [Å] | 3.200 | 3.200 |
Rmerge | 0.079 | 0.256 |
Number of reflections | 7481 | |
<I/σ(I)> | 7.9 | 2.9 |
Completeness [%] | 99.5 | 98 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.9 | 293 | 5-8 mg/ml protein 30-45% MPD 0.2-0.4 M Ammonium Sulfate Tris, 15mM KCl 75mM 5% glycerol 5-10mM magnesium Chloride 1-5 mM ATP , pH 7.9, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
1 | VAPOR DIFFUSION, HANGING DROP | 7.9 | 293 | 5-8 mg/ml protein 30-45% MPD 0.2-0.4 M Ammonium Sulfate Tris, 15mM KCl 75mM 5% glycerol 5-10mM magnesium Chloride 1-5 mM ATP , pH 7.9, VAPOR DIFFUSION, HANGING DROP, temperature 293K |