2EWU

The F20H mutant of tetraheme cytochrome c3 from Desulfovibrio Vulgaris Miyazaki F

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL44B2
Synchrotron site	SPring-8
Beamline	BL44B2
Temperature [K]	100
Detector technology	CCD
Collection date	2004-11-15
Detector	ADSC QUANTUM 4
Wavelength(s)	0.7
Spacegroup name	P 21 21 21
Unit cell lengths	52.196, 67.418, 34.454
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	10.000 - 1.100
R-factor	0.1113
Rwork	0.111
R-free	0.15300
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.018
RMSD bond angle	0.033
Data reduction software	HKL-2000
Data scaling software	CCP4 ((SCALA))
Phasing software	SHELX
Refinement software	SHELXL-97

Data quality characteristics

	Overall
Low resolution limit [Å]	15.900
High resolution limit [Å]	1.100
Number of reflections	50149

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	7.4	277	40% MPD, pH 7.4, VAPOR DIFFUSION, temperature 277K