2EWR
Crystal structure of a putative nucleotidyltransferase (tm1012) from Thermotoga maritima at 1.60 A resolution
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-D |
| Synchrotron site | APS |
| Beamline | 23-ID-D |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-08-04 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 0.95372, 0.97960, 0.97942 |
| Spacegroup name | P 1 |
| Unit cell lengths | 32.560, 36.210, 39.280 |
| Unit cell angles | 74.11, 86.38, 79.69 |
Refinement procedure
| Resolution | 29.660 - 1.600 |
| R-factor | 0.14658 |
| Rwork | 0.145 |
| R-free | 0.18000 |
| Structure solution method | MAD |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.486 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 29.643 | 1.660 | |
| High resolution limit [Å] | 1.600 | 3.440 | 1.600 |
| Rmerge | 0.028 | 0.134 | 0.095 |
| Number of reflections | 20185 | 4384 | 2364 |
| <I/σ(I)> | 18.43 | 30.94 | 6.96 |
| Completeness [%] | 88.8 | 50.4 | |
| Redundancy | 3.75 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP, NANODROP | 6.9 | 277 | 0.2M KCl, 20.0% PEG-3350, No Buffer, pH 6.9, VAPOR DIFFUSION, SITTING DROP, NANODROP, temperature 277K |
