2EWO
X-ray structure of putative agmatine deiminase Q8DW17, Northeast Structural Genomics target SmR6.
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X4A |
Synchrotron site | NSLS |
Beamline | X4A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2005-08-25 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.97938 0.97952 0.96793 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 92.524, 203.685, 139.543 |
Unit cell angles | 90.00, 104.72, 90.00 |
Refinement procedure
Resolution | 19.990 - 2.900 |
R-factor | 0.235 |
Rwork | 0.235 |
R-free | 0.26900 |
Structure solution method | MAD |
RMSD bond length | 0.017 |
RMSD bond angle | 1.800 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | SOLVE |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.900 |
High resolution limit [Å] | 2.800 | 2.800 |
Rmerge | 0.145 | 0.696 |
Number of reflections | 218714 | |
<I/σ(I)> | 9.1 | 1.99 |
Completeness [%] | 99.8 | |
Redundancy | 4.2 | 3.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 9.1 | 294 | 16% PEG6K, 100mM Tris-HCl, 400mM LiCl, pH 9.1, VAPOR DIFFUSION, HANGING DROP, temperature 294K |