2EWI
The F20Y mutant of tetraheme cytochrome c3 from Desulfovibrio Vulgaris Miyazaki F
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL44B2 |
| Synchrotron site | SPring-8 |
| Beamline | BL44B2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-06-16 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.71 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 52.242, 67.483, 34.328 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 10.000 - 1.000 |
| R-factor | 0.112 |
| Rwork | 0.112 |
| R-free | 0.15000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.021 |
| RMSD bond angle | 0.037 |
| Data reduction software | HKL-2000 |
| Data scaling software | CCP4 ((SCALA)) |
| Phasing software | SHELX |
| Refinement software | SHELXL-97 |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 21.900 |
| High resolution limit [Å] | 1.000 |
| Number of reflections | 62471 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 7.4 | 277 | MPD 70%, pH 7.4, VAPOR DIFFUSION, temperature 277K |
