2EWH
Carboxysome protein CsoS1A from Halothiobacillus neapolitanus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.2.2 |
| Synchrotron site | ALS |
| Beamline | 8.2.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-10-21 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.00000 |
| Spacegroup name | P 6 |
| Unit cell lengths | 66.417, 66.417, 28.982 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 57.540 - 1.400 |
| R-factor | 0.187 |
| Rwork | 0.185 |
| R-free | 0.24000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2a10 |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.691 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 90.000 | 1.450 |
| High resolution limit [Å] | 1.400 | 1.400 |
| Rmerge | 0.072 | 0.395 |
| Number of reflections | 13685 | |
| Completeness [%] | 94.1 | 71.8 |
| Redundancy | 8.8 | 5.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 9.5 | 293 | 30% PEG 400, 0.1M 2(cyclohexylamino)ethanosulfonic acid, pH 9.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
