2EW2
Crystal Structure of the Putative 2-Dehydropantoate 2-Reductase from Enterococcus faecalis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-10-08 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9794 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 89.165, 89.160, 104.754 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 33.950 - 2.000 |
| R-factor | 0.18452 |
| Rwork | 0.181 |
| R-free | 0.21442 |
| Structure solution method | SAD |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.204 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | HKL-3000 |
| Refinement software | REFMAC (5.2.0000) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 33.950 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.089 | 0.386 |
| Number of reflections | 53058 | |
| <I/σ(I)> | 10.6 | 4.16 |
| Completeness [%] | 92.9 | 59.8 |
| Redundancy | 11.7 | 9.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 289 | 100mM Tris/HCL, 30% PEG400, 200mM MgCL2, pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 289K |
