2EVW
Crystal structure analysis of a fluorescent form of H-Ras p21 in complex with R-caged GTP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID14-4 |
Synchrotron site | ESRF |
Beamline | ID14-4 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2004-12-01 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.939 |
Spacegroup name | P 41 |
Unit cell lengths | 69.300, 69.300, 35.510 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 69.340 - 1.050 |
R-factor | 0.157 |
Rwork | 0.156 |
R-free | 0.18100 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5p21 |
RMSD bond length | 0.015 |
RMSD bond angle | 1.855 |
Data scaling software | XSCALE |
Phasing software | MOLREP |
Refinement software | REFMAC |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 69.340 | 1.050 |
High resolution limit [Å] | 1.000 | 1.000 |
Rmerge | 0.052 | 0.679 |
Number of reflections | 78838 | 11588 |
<I/σ(I)> | 13.82 | 2.43 |
Completeness [%] | 99.9 | 99.9 |
Redundancy | 5.9 | 4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.2 | 291.15 | protein solution: 64 mM TRIS pH 7.6, 20 mM magnesium chloride, 10 mM DTT, 0,1 mM sodium azide; reservoir solution: 100 mM HEPES pH 7.2, 200 mM magnesium acetate, 16% PEG 8000 (freshly prepared) mixture of equal volumes of protein and reservoir solution, VAPOR DIFFUSION, HANGING DROP, temperature 291.15K |