2EVV
Crystal Structure of the PEBP-like Protein of Unknown Function HP0218 from Helicobacter pylori
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-BM |
| Synchrotron site | APS |
| Beamline | 19-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-04-03 |
| Detector | SBC-3 |
| Wavelength(s) | 0.97922 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 102.312, 102.266, 143.047 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 45.750 - 2.590 |
| R-factor | 0.2235 |
| Rwork | 0.219 |
| R-free | 0.26248 |
| Structure solution method | SAD |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.441 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | HKL-3000 |
| Refinement software | REFMAC (5.2.0000) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.750 | 2.680 |
| High resolution limit [Å] | 2.590 | 2.590 |
| Rmerge | 0.191 | 0.798 |
| Number of reflections | 47122 | |
| <I/σ(I)> | 4.9 | 2.2 |
| Completeness [%] | 97.6 | 79.7 |
| Redundancy | 11.1 | 8.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 295 | 0.1M Bis-Tris PH6.0, 0.2M Ammonium Sulphate, 20%PEG4K, 2% Glycerol, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
