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2EVU

Crystal structure of aquaporin AqpM at 2.3A resolution

Experimental procedure
Experimental methodSINGLE WAVELENGTH
Source typeSYNCHROTRON
Source detailsALS BEAMLINE 8.3.1
Synchrotron siteALS
Beamline8.3.1
Temperature [K]100
Detector technologyCCD
Collection date2003-11-23
DetectorADSC QUANTUM 4
Wavelength(s)1.1
Spacegroup nameI 4
Unit cell lengths93.887, 93.887, 77.744
Unit cell angles90.00, 90.00, 90.00
Refinement procedure
Resolution33.200 - 2.300
R-factor0.188
Rwork0.188
R-free0.22600
Structure solution methodMOLECULAR REPLACEMENT
RMSD bond length0.007
RMSD bond angle1.100
Data reduction softwareDENZO
Data scaling softwareSCALEPACK
Phasing softwareCNS
Refinement softwareCNS
Data quality characteristics
 OverallOuter shell
Low resolution limit [Å]50.0002.370
High resolution limit [Å]2.3002.300
Number of reflections15208
Completeness [%]94.486
Crystallization Conditions
crystal IDmethodpHtemperaturedetails
1VAPOR DIFFUSION, HANGING DROP929520% PEG 4000, 50 mM TRIS pH 9, 100 mM NaCl, 10% glycerol, pH 9.0, VAPOR DIFFUSION, HANGING DROP, temperature 295K

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