2EVS

Crystal structure of human Glycolipid Transfer Protein complexed with n-hexyl-beta-D-glucoside

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RUH3R
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2005-05-30
Detector	RIGAKU RAXIS HTC
Wavelength(s)	1.5418
Spacegroup name	C 1 2 1
Unit cell lengths	157.913, 42.180, 70.819
Unit cell angles	90.00, 104.57, 90.00

Refinement procedure

Resolution	15.000 - 2.200
R-factor	0.192
Rwork	0.190
R-free	0.23600
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1swx
RMSD bond length	0.014
RMSD bond angle	1.467
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	REFMAC

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.280
High resolution limit [Å]	2.200	2.200
Rmerge	0.080	0.452
Number of reflections	22940
<I/σ(I)>	8.7
Completeness [%]	98.6	98.1
Redundancy	3.9	3.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	4.5	293	15-20% PEG 3350, potassium phosphate, pH 4.5, VAPOR DIFFUSION, temperature 293K