2EVJ
Structure of an Ndt80-DNA complex (MSE mutant mA9C)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 95 |
Detector technology | CCD |
Collection date | 2001-12-08 |
Detector | SBC-2 |
Wavelength(s) | 1.0093 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 69.626, 79.039, 161.684 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 35.580 - 1.890 |
R-factor | 0.174 |
Rwork | 0.172 |
R-free | 0.20200 |
Structure solution method | FOURIER SYNTHESIS |
Starting model (for MR) | PDB 1MNN - MINUS DNA AT SEQUENCE CHANGES AND 1 BASEPAIR ADJACENT TO THOSE CHANGES |
RMSD bond length | 0.008 |
RMSD bond angle | 1.269 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Refinement software | REFMAC |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 81.650 | 1.970 |
High resolution limit [Å] | 1.890 | 1.890 |
Number of reflections | 35750 | |
Completeness [%] | 99.9 | 100 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 295 | 30% PEG 400, 50 mM bis-tris-propane pH 7.0, 100 mM NaCl, 50 mM CaCl2, and 2 mM DTT. 1:1 Molar ratio protein:DNA, protein at 20mg/ml, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
Crystallization Reagents
ID | crystal ID | solution ID | reagent name | concentration | details |
1 | 1 | 1 | PEG 400 | ||
2 | 1 | 1 | bis-tris-propane | ||
3 | 1 | 1 | NaCl | ||
4 | 1 | 1 | CaCl2 | ||
5 | 1 | 1 | DTT | ||
6 | 1 | 1 | H2O | ||
7 | 1 | 2 | PEG 400 | ||
8 | 1 | 2 | NaCl | ||
9 | 1 | 2 | CaCl2 |