2EVE
X-Ray Crystal Structure of Protein PSPTO5229 from Pseudomonas syringae. Northeast Structural Genomics Consortium Target PsR62
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X4A |
| Synchrotron site | NSLS |
| Beamline | X4A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-10-27 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.9791 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 77.233, 42.273, 50.655 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.710 - 1.600 |
| R-factor | 0.191 |
| Rwork | 0.191 |
| R-free | 0.21700 |
| Structure solution method | SAD |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.100 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | SnB |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.710 | 1.610 |
| High resolution limit [Å] | 1.550 | 1.550 |
| Rmerge | 0.086 | 0.394 |
| Number of reflections | 46260 | |
| <I/σ(I)> | 23.75 | 8.48 |
| Completeness [%] | 99.2 | 98.6 |
| Redundancy | 14.1 | 14.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 0.5mM protein, 100mM MOPS, 30% PEG8000, 100mM NaH2PO4, 5mM DTT, pH 7, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
