2EVC

Crystal structure of E. Coli. methionine amino peptidase in complex with 5-(2-(trifluoromethyl)phenyl)furan-2-carboxylic acid

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RUH3R
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2004-11-08
Detector	RIGAKU RAXIS IV
Wavelength(s)	1.5418
Spacegroup name	P 1 21 1
Unit cell lengths	37.800, 60.200, 50.400
Unit cell angles	90.00, 104.50, 90.00

Refinement procedure

Resolution	20.000 - 1.600
R-factor	0.22
Rwork	0.210
R-free	0.22900
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2mat
RMSD bond length	0.005
RMSD bond angle	1.330
Data reduction software	XDS
Data scaling software	XDS
Phasing software	AMoRE
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	1.700
High resolution limit [Å]	1.600	1.600
Number of reflections	26974
Completeness [%]	93.1	89.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	7.5	292	15% PEG 8000, 0.1M HEPES, pH 7.5, VAPOR DIFFUSION, temperature 292K