2EV6
Bacillus subtilis manganese transport regulator (MNTR) bound to zinc
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.2.1 |
Synchrotron site | ALS |
Beamline | 8.2.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2004-07-11 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 1.0781 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 49.710, 46.000, 74.390 |
Unit cell angles | 90.00, 93.17, 90.00 |
Refinement procedure
Resolution | 37.140 - 1.700 |
R-factor | 0.233 |
Rwork | 0.233 |
R-free | 0.26000 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1on1 |
RMSD bond length | 0.005 |
RMSD bond angle | 1.000 |
Data reduction software | MOSFLM |
Data scaling software | CCP4 ((SCALA)) |
Phasing software | CNS |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 37.140 | 1.810 |
High resolution limit [Å] | 1.700 | 1.700 |
Rmerge | 0.077 | 0.328 |
Number of reflections | 34860 | |
<I/σ(I)> | 5.6 | 2.2 |
Completeness [%] | 93.6 | 72.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 295 | 0.1M sodium citrate, 0.2M ammonium citrate, 20% PEG 3000, 5mM ZnCl2, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 295K |