2EV5
Bacillus subtilis manganese transport regulator (MNTR) bound to calcium
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.2.1 |
Synchrotron site | ALS |
Beamline | 8.2.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2004-07-11 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 1.0781 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 49.690, 45.770, 74.730 |
Unit cell angles | 90.00, 93.76, 90.00 |
Refinement procedure
Resolution | 39.000 - 2.000 |
R-factor | 0.248 |
Rwork | 0.248 |
R-free | 0.29100 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1on1 |
RMSD bond length | 0.006 |
RMSD bond angle | 1.000 |
Data reduction software | MOSFLM |
Data scaling software | CCP4 ((SCALA)) |
Phasing software | CNS |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 39.000 | 2.130 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.109 | 0.278 |
Number of reflections | 21041 | |
<I/σ(I)> | 4.4 | |
Completeness [%] | 91.8 | 91.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 295 | 0.1M Tris HCl, 0.2M calcium acetate, 18% PEG 8000, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 295K |