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2EUX

Structure of a Ndt80-DNA complex (MSE VARIANT vA4G)

Experimental procedure
Experimental methodSINGLE WAVELENGTH
Source typeSYNCHROTRON
Source detailsALS BEAMLINE 8.3.1
Synchrotron siteALS
Beamline8.3.1
Temperature [K]105
Detector technologyCCD
Collection date2003-07-14
DetectorADSC QUANTUM 210
Wavelength(s)1.072158
Spacegroup nameC 2 2 21
Unit cell lengths69.955, 79.133, 161.832
Unit cell angles90.00, 90.00, 90.00
Refinement procedure
Resolution35.580 - 1.570
R-factor0.173
Rwork0.171
R-free0.20300
Structure solution methodFOURIER SYNTHESIS
Starting model (for MR)PDB 1MNN - MINUS DNA AT SEQUENCE CHANGES AND 1 BASEPAIR ADJACENT TO THOSE CHANGES
RMSD bond length0.010
RMSD bond angle1.453
Data reduction softwareMOSFLM
Data scaling softwareSCALA
Refinement softwareREFMAC
Data quality characteristics
 OverallInner shellOuter shell
Low resolution limit [Å]38.44053.9401.650
High resolution limit [Å]1.5704.9601.570
Rmerge0.0510.0370.225
Number of reflections59499
<I/σ(I)>8.816.73
Completeness [%]94.69872.2
Redundancy4.36.21.9
Crystallization Conditions
crystal IDmethodpHtemperaturedetails
1VAPOR DIFFUSION, HANGING DROP729530% PEG 400, 50 mM bis-tris-propane pH 7.0, 100 mM NaCl, 50 mM CaCl2, and 1 mM DTT. 1:1 Molar ratio protein:DNA, protein at 10mg/ml , VAPOR DIFFUSION, HANGING DROP, temperature 295K
Crystallization Reagents
IDcrystal IDsolution IDreagent nameconcentrationdetails
111PEG 400
211bis-tris-propane
311NaCl
411CaCl2
511DTT
611H2O
712PEG 400
812NaCl
912CaCl2

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PDB entries from 2024-11-13

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