2EUL
Structure of the transcription factor Gfh1.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2005-09-14 |
| Detector | RIGAKU RAXIS IV |
| Spacegroup name | P 43 |
| Unit cell lengths | 59.291, 59.291, 218.876 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 - 2.400 |
| R-factor | 0.206 |
| Rwork | 0.206 |
| R-free | 0.24500 |
| Structure solution method | MIR |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.450 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | CCP4 |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.480 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.040 | 0.482 |
| Number of reflections | 29039 | |
| <I/σ(I)> | 35.6 | 8.2 |
| Completeness [%] | 91.4 | 58.8 |
| Redundancy | 7.8 | 4.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.5 | 277 | 8% PEG800, 33mM ZnAc, 17mM NaCacodylate, 0.85M NaCl, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 277K, pH 6.50 |
